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As in previous versions of FileMaker, provision is made for scripts to be assigned to run OnFileOpen and OnFileClose. This capability has not changed in FileMaker Pro 10, and the opening and closing scripts are defined by accessing the Open/Close tab of the File Options dialog (choose File File Options). Although the dialog options for the file-based script triggers refer to opening and closing the file, in reality, the OnFileOpen script doesn t run until the first window is displayed. Thus, when a file is opened hidden (either via script or as a consequence of as an external data source for another open file), the opening script will not be called. Only when and if a window is opened onscreen will the opening script be triggered. Thus, the OnFileOpen script trigger might more properly be considered an OnFirstWindowDraw trigger. Similarly, because a file that is referenced by another open file won t close until all the files referencing it also close, but the OnFileClose trigger will fire when the last window associated with the file is closed, the OnFileClose trigger might be better considered an OnLastWindowClose trigger

Another common use of information is as the basis of correspondence. Letters to colleagues, associates, customers, or clients usually contain information that is relevant and specific to the recipient. These letters can be produced from a database as a kind of report one in which the elements of data and/or summary information are arranged within appropriate text, in a format that is conventional for correspondence. In this way, using the data that is already in your database, you can efficiently create dozens, or even hundreds, of different letters each specific to the addressee. This particular type of correspondence, sometimes called a form letter, is a common feature of word-processing applications, such as Microsoft Word. In Word, this feature is called Data Merge, and you use it to retrieve data from a separate merge data file (such as an Excel or Access file). FileMaker Pro lets you create such correspondence without involving other applications. By enabling you to enter your data once and then retrieve it in a variety of configurations and formats (as screens, forms, reports, summaries, lists, or letters), a database turns unwieldy tables of data into a flexible and powerful tool.

1. In Layout mode, choose Layouts Set Tab Order. The Set Tab Order dialog appears. A numbered arrow marks each field in the layout. (See Figure 6-44.) 2. If you wish to add Objects (fields, tab controls, and buttons), only fields, or only buttons to the tab order or if you want to remove fields or buttons from the existing tab order choose from the Add Remaining and Remove pop-up menus/drop-down lists, respectively.

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Stage 2 (Day 4). (a) After 4 days of culture in suspension, remove the embryoid bodies to a 15-mL tube and allow to settle under gravity for about 15 min. (b) Aspirate the old medium and replace with 5 mL of neurosphere expansion medium. (c) Place approximately 50 EBs into each of several gelatin-coated 25-cm2 asks and make the total amount of neurosphere expansion medium up to 10 mL per ask. (d) Place the asks in a 5% CO2 incubator at 37 C for 4 days. (e) Aspirate the old medium and replace it with 10 mL of new neurosphere expansion medium every 2 days. Stage 3 (Day 14). (a) After 10 days at stage 2, check the asks under a phase-contrast microscope for the presence of neural rosettes (Fig. 3.1). (b) Aspirate the supernate from each ask and add 1 mL of 0.1 mg/mL Dispase to each. Ensure that the dispase covers the cell layer before placing the ask in a 5% CO2 incubator at 37 C for 15 min. (c) Remove the ask from the incubator and gently sway from side to side in order to encourage disaggregated neural rosettes to detach. (d) Gently add 9 mL of DMEM/F12 to each ask to dilute the dispase and remove the 10 mL (total) with neural rosette clusters to a 15-mL tube. (e) Centrifuge the tube at 800 rpm (123 g) for 3 min at 4 C, then remove the supernate and resuspend the pellet in 5 mL of neurosphere medium.

The next method in the VideoPlayer class is getCategories(), which is called from the constructor when the application initializes. This method starts by creating a new instance of the Responder, which handles the response from the remoting service.

Reagents and Materials Sterile or aseptically prepared Culture of EC stem cells, 25 cm2 or 75 cm2 (see Protocol 6.2) Freezing medium (see Section 6.2.3) Nonsterile Insulated box for freezing Ultra deep-freeze at 70 C Liquid nitrogen freezer or equivalent Procedure